This is what you will receive before customizing this microscope:
Standard Leica DM5500 B Microscope Configuration:
Microscopy Techniques:
Brightfield with Phase Contrast and Fluorescence Microscopy
Base:
Leica DM5500 B Upright Microscope
Motorized Z Focus
Microscope Control:
CTR5500 Control Box/Power Supply
Head:
Trinocular Head
(2) HC PLAN s 10x/22 M Eyepieces
Prisms / Condensers:
0.9 S1 Motorized Smart Condenser
Ph1 Prism
Brightfield Path:
Base
Fluorescence Light Path:
Collimating Adapter w/ light guide
Fluorescence filter cube turret:
8-position Motorized Turret
Fluorescence Filters:
Three filter cubes
Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
*Other filters available - please inquire* Illumination:
12V 100W Halogen Lamp w/ CTR5500 power supply
EXFO X-Cite 120 PC Metal Halide Light Source w/ collimating adapter and light guide
Stage:
XY Mechanical and Rotatable Stage
Objectives:
7-position Mechanical Turret
5x/0.12 N PLAN PH0 (O3720)
10x/0.25 HCX FL PLAN PH1 (O3721)
20x/0.50 HCX PL FLUOTAR (O3724)
*Other objectives available - please inquire* Photo / Port Splits:
100% Eye
50/50 Eye/Cam
100% Camera
Camera:
10MP CMOS Camera
Computer and Software:
Computer running MicroManager and applicable camera software
Communication and power cables.
Motorized Components:
Filter Cube Turret
Halogen Light Intensity
Fluorescence Shutter
Halogen Aperture and Field Diaphragm
Fluorescence Aperture and Field Diaphragm
Z-axis
This configuration includes any 3 of these new filter sets.
New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)
Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
Motorized/automated Z-Axis Drive
Motorized filter cube turret
Benefits of motorized/Automated Z-Axis drive:
Automatic parfocality adjustment
The ability to recall previously saved focus levels
Automated management of Z positioning/ Fine-coarse control
This microscope's imaging capabilities:
Imaging software highlights:
Ability to take still images and live videos of your samples
Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
Variable linear measurements such as line segment, radius values, and others
Multi-slice (Z coordinate/Z Stacking)
Multi-frame (Time lapse/Image acquisition over time)
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)
DIC or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
We can add standard higher magnification Leica microscope objectives
We can add high-end Leica objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
Additional filter cubes
Additional filter sets to match your fluorochromes.
Xenon or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
Add other Motorized/Automation Capabilities:
In addition to installed motorized capabilities, the microscope can be configured
Motorized Stage, Motorized high-speed shutter
We can configure it to work with the sample medium you need:
Slide, Petri dish, microplate, etc
It may also be set up with a motorized XY stage for multi-slide or full microplate scans.
Commonly Requested Customer Upgrades
High-End CCD, sCMOS Cameras
LED or Metal Halide Fluorescence Light Sources
Phase Contrast, Polarization, or the DIC configurations
Higher Magnification Objectives
Higher performance Fluor or APO objectives
Motorized XY Stage (stage, controller, joystick)
We are excited to help build and configure the right microscope for you!
Leica DM5500 B Fluorescence Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
The Leica Leica DM5500 B is an inverted microscope renowned for its exceptional imaging capabilities in life science research. Equipped with high-performance optics, this instrument offers outstanding image quality, enabling researchers to visualize biological specimens with unparalleled clarity and precision. With a versatile range of observation techniques, including brightfield, phase contrast, and fluorescence imaging, the DM5500 B facilitates detailed examination of cellular structures and dynamics. Overall, the Leica DM5500 B is a powerful tool for researchers seeking to explore the complexities of cellular biology with unparalleled imaging performance and flexibility.
Leica DM5500 B is suitable for the following applications as configured:
Fluorescence microscopy: Ideal for fluorescent labeling and imaging of cells and tissues.
Material science research: Suitable for examining material properties and structures.
Biological research: Essential for cellular structure, function, and behavior studies.
Pharmacological studies: Facilitates the investigation of drug effects on cells and tissues.
Biotechnology applications: Enables genetic engineering and molecular biology research.
Educational purposes: Serves as a teaching tool in universities and research institutes.
Phase contrast: Enhances visibility of transparent specimens without staining.
Time-lapse photography: Captures sequential images to monitor changes over time.
Multi-dimensional imaging: Allows for the acquisition of images in multiple planes and dimensions.
Other potential applications:
DIC: Enhances visibility of transparent specimens without staining.
High-resolution imaging: Provides clear, detailed images for in-depth analysis of samples.
We make sure to match the microscope capabilities to your requirements.
We have experience servicing and repairing these microscopes.
This microscope goes through an extensive set of service steps before shipping.
We offer detailed photos and videos of the microscopes you buy.
We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
Many upgrade and repair parts are in stock.
Remote installation and microscope operation training are available.
On-site installation may be available in the Boston Area (New England area)
Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
Warranty
An extended warranty may be available.
You get after-sales technical support.
Customer references are available.
The microscope will be complete and working!
Smooth process from the initial quote to order fulfillment.
*SM* Small Crate
200#
COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW
What steps do you take to verify, service, and confirm the functionality of the Leica DM5500 B microscope?
We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.
We confirm the microscope's physical condition.
We meticulously inspect and clean the optical path within the microscope.
We lubricate mechanical components.
We assess light source quality using various fluorescence and non-fluorescence samples.
We ensure the stage moves smoothly in both fine and coarse adjustments.
We ensure the smooth operation of all mechanical parts.
We confirm that all electronic/motorized components are working well.
We set up communication between the microscope, installed components, and the software.
We test the camera by acquiring sample images.
We confirm the responsiveness of any included touchscreens and buttons.
We ensure necessary accessories and cables are included.
Can you help me identify the best microscope and configuration for me?
We are here to help.
Contact us with your application; we will help you identify the best microscope configuration.
We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.
Can I view the Leica DM5500 B microscope in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
The microscope is fully warranted for parts and labor.
The customer should retain all packing materials during the warranty period.
Installation & Training:
Option 1 – In Person
If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.
Option 2 – Remote/Virtual
Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.
Both training options include:
Review of the microscope and its capabilities.
Microscope and software operation.
Review of the recommended maintenance procedures.
We generally recommend these training services to anyone who is new to this microscope model.
What should I watch out for when buying a Leica DM5500 B microscope used/pre-owned from another lab?
Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
Confirm that there is no physical damage or any misaligned internal components.
Ask what accessories are included.
Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.
We confirm the microscope's physical condition.
We meticulously inspect and clean the optical path within the microscope.
We assess light quality using various fluorescence and non-fluorescence samples.
We ensure the stage moves smoothly in both fine and coarse adjustments.
We ensure the smooth operation of all mechanical components.
We confirm that all electronic/motorized components are working well.
We set up communication between the microscope, installed components, and the software.
We test the camera by acquiring sample images.
We confirm the responsiveness of any included touchscreens and buttons.
We ensure necessary accessories and cables are included.
We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.
Do you have a Leica DM5500 B microscope on hand?
Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model microscope elsewhere, possibly for less.
Here are some of the reasons for our price.
Our microscope will be fully serviced.
It will be customized and configured to your requirements.
We may offer longer standard warranty terms.
We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
We invest considerable time into the testing and service of each microscope we sell.
We have to purchase any missing required accessories to make the microscopes complete.
We have to procure and stock service parts to fulfill our warranty.
We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
We guarantee that the microscope will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
POs are accepted from US and Canadian universities.
How fast will you ship?
We can ship within a few days to 2 weeks, depending on your required microscope customization.
Will my microscope be insured during shipping?
All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you previously packed and shipped a Leica DM5500 B microscope? Do you ship Internationally?
Packing
Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
Accessories are packed safely in boxes with padding and then safely packed inside the crate.
We may not use a crate for local deliveries to save you money *if delivered by us.
We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile microscopes globally.
Our experience and process
We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
Know that your microscope was tested and working the day it left our facility and is covered if there are faults.
Sustainability
Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
Brochure for Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope DM5500B Pred DM4/DM6 - AV
(Click on the brochure below to scroll thru it)
(Click on the empty blank/white space to scroll thru the microscope listing)
Here we are showing you our standard packing process
1. All items are different, but they all follow the same principle when packaging.
2. Start with a well-constructed crate
A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.
3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.
4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.
5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.
6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.
7. Final step is to photograph everything inside the crate and then seal it with screws.
All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to [email protected] or call (617) 397-3486.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.