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Nikon E400 Fluorescence Microscope Unit2 Pred Ni - AV

Nikon E400 Fluorescence Microscope Unit2 Pred Ni - AV
Nikon E400 Fluorescence Microscope Unit2 Pred Ni - AV
  • Stock: In Stock
  • Brand: Nikon
  • Product Code: 42307

Here is a Nikon E400 Fluorescence Microscope Pred Ni Unit2

Setup for Brightfield with Fluorescence studies.

- This microscope can be used in biological studies
- Enhancing components, such as Fluorescence Filter Cubes have been added (Other components are available)
- The total current magnification range of this microscope is 40 to 200x (Other objectives are available)
- This microscope is compatible with slides. (Options for petri dishes and microplates may be available)
- The fluorescence light source included is an HBO which uses inexpensive replacement lamps and will arrive with a brand new bulb (installation required)
- The included camera software allows for live viewing and image/video capture with variable linear measurements such as line segment, radius values, and others
- The easy to use interface also allows for customization of color and resolutions settings

Predecessor to:
Current model is Ni

This Nikon E400 Fluorescence Microscope is in good working order with all aspects tested to be working fully.
Microscope optics have been professionally cleaned under a class 10,000 clean room hood.

Please see the exact video and photos below.

Testing we performed:
The Nikon E400 Fluorescence Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source fully: PASSED
Tested fluorescence and source fully: PASSED
Tested transmission halogen light path: PASSED
Tested reflective fluorescence light path: PASSED
Tested objectives for clarity: PASSED
Tested port-splitter to split between camera and eyepieces: PASSED
Tested mechanical condenser: PASSED
Tested mechanical Z-axis: PASSED
Tested mechanical fluorescence turret: PASSED
Tested mechanical objective turret: PASSED
Tested connection from camera to laptop: PASSED
Tested mechanical stage: PASSED
Tested live feed for clarity: PASSED
Tested all filter slides: PASSED

During testing the Nikon E400 Fluorescence Microscope was found to be working fully.
A clear image was produced both through the eyepieces and camera port.
Test sample was able to be focused without excessive effort.

System consists of:
Nikon Eclipse E400 Upright Microscope
Trinocular Head
(2) CFI 10X/22 Eyepieces
0.90 Condenser
Brightfield Path:
Fluorescence Path:
Y-FL Fluorescence Path
Fluorescence Filter Cubes:
4-position Filter Slider
TRITC (Green) EX: 540/25 DM: 565 EM: 620/60
B2A (Blue) EX: 450-490 DM: 505 EM: 520
GFP BP (Green) EX: 480/40 DM: 505 EM: 535/40
*Other filters available - please inquire*
6V 30W Halogen Lamp w/ built-in power supply
100W Mercury Lamp w/ HB-10103AF power supply
5-position Mechanical Nosepiece
4x/0.13 Plan Fluor (O#003816)
10x/0.30 Plan Fluor (O#003821)
20x/0.75 CFI Plan Apo VC (O#003145) (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
*Other objectives available - please inquire*
Mechanical XY
Port Splits:
100% Eye or 100% Camera
3MP CMOS Camera
Computer and Software:
Computer running applicable software

Communication and power cables.

Configuration and Setup:
Orientation: Upright
Illumination Type: Brightfield w/ Fluorescence
Objectives: 4x/0.13 Plan Fluor, 10x/0.30 Plan Fluor, 20x/0.75 CFI Plan Apo VC
Light Sources: 6V 30W Halogen Lamp, 100W Mercury Lamp
Stage: Mechanical XY

This Nikon E400 Fluorescence Microscope is currently set up for Brightfield with Fluorescence studies.
The CMOS camera is capable of taking detailed digital images.
With included objectives of 4x, 10x, 20x alongside with 10x eyepieces the current total magnification range is 40x to 200x

Video Description:
*This video shows the actual microscope in this listing.*

The video provides a walk around view of this Nikon E400 Fluorescence Microscope and its included components.
A functionality demonstration has been performed to show proper operation of the Nikon E400 Fluorescence Microscope.

Any available additional options functionality has also been demonstrated.

The camera that will be included with this microscope may differ from the one shown in the demonstration.

This will ship via freight. Please contact us with your zip code to obtain shipping quote.

*SM* Small Crate

Please do not hesitate to contact us if you have any questions.


Brochure for Nikon E400 Fluorescence Microscope Unit2 Pred Ni - AV
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Manual for Nikon E400 Fluorescence Microscope Unit2 Pred Ni - AV

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Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.


All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to or call (617) 397-3486.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

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