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Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7

Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
Zeiss AXIO Observer Inverted LED Fluorescence Motorized XY Definite Focus Microscope (New Filters) Pred 7
  • Stock: In Stock
  • Brand: Zeiss
  • Product Code: SM100331-DEFXYAURA
$20,995.00
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For Sale Zeiss AXIO Observer Z1 Inverted LED Fluorescence Motorized XY with Definite Focus Microscope (New Filters) Pred Observer 7

 

Pre-owned microscope (this microscope can be fully customized)

 

  • Fully Functional
  • Serviced & in Stock
  • Good cosmetic condition
  • Comes with Brand New Fluorescence Filter Sets
  • Continental US Warranty 120 Days & 60 Days International.
  • The training package is available.
  • Renewable single or multi-year warranty agreements are available.

 

The Predecessor to Zeiss Observer 7 Inverted Fluorescence Microscope

 

This is what you will receive before customizing this microscope:

Standard Zeiss AXIO Observer Z1 LED Fluorescence Microscope with Definite Focus Configuration:

 

Microscopy Techniques:

Brightfield and Fluorescence Microscopy

 

Microscope Base:

  • Zeiss Observer Z1 Base with camera port
  • Motorized(Automated) Z-Axis Drive
  • With Zeiss Touch Controller

Microscope Head:

  • Binocular Head 
  • Two Eyepieces

Fluorescence Light Path:

  • Zeiss fluorescence lightpath built into the base

Fluorescence Illumination:

  • Lumencor LED Aura 5 Color Light Engine
  • Light guide
  • Colliminating adapter

Fluorescence filter cube turret:

  • Motorized 5-position filter cube turret

Fluorescence filters:

  • Three filter cubes
  • Three Brand New filter sets of either DAPI, FITC, Texas Red, CY5, CY3

Brightfield Light Path:

  • Illumination Tower

Brightfield Illumination:

  • 100 Watt halogen light source
  • 100 Watt halogen power supply built into the base

Objective Turret:

  • Motorized 6-position objectives turret
  • Zeiss Definite Focus Drift System with Controller

Objectives:

  • 5x/0.15 Plan-Neofluar
  • 10x/0.30 Plan-Neofluar
  • 20x/0.40 Plan-Neofluar

Condenser:

  • Motorized condenser

Stage:

  • Motorized Stage XY
  • Controller
  • Joystick

Camera ports:

  • 100% Eyepiece, 0% Camera
  • 20% Eyepiece, 80% Camera
  • 0% Eyepiece, 100% Camera

Camera adapter:

  • 1x C-mount adapter 

Camera:

  • CMOS USB 3.0 10MP Camera

Computer & Software:

  • Dell laptop computer with Windows 10
  • Micromanager w/ImageJ analysis software
  • Camera software

Accessories:

  • Necessary power and communication cables

 

This configuration includes any 3 of these new filter sets.

  • New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
  • New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
  • New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
  • New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
  • New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

 

Automated/motorized capabilities of this microscope:

Installed automation/motorized components:

  • Motorized lightpath
  • Motorized/automated Z-Axis Drive
  • Motorized filter cube turret
  • Motorized objective(nosepiece) turret
  • Motorized Condenser
  • Motorized XY Stage
  • Definite Focus Drift Control System

 

Benefits of motorized/Automated Z-Axis drive:

  • Automatic parfocality adjustment
  • The ability to recall previously saved focus levels
  • Automated management of Z positioning/ Fine-coarse control

 

How the Nikon Perfect Focus System Works

  • Infrared Light Technique: Infrared light that doesn't affect the specimen is used to detect focal shifts.
  • Real-Time Monitoring: Continually checks and adjusts the focal plane to maintain sharp imaging.
  • Sensor-Based Feedback: Employs sensors to measure deviations from the desired focus point.
  • Automated Corrections: Adjusts the position of the microscope Z-axis to correct any focus drift automatically.
  • Integration with Imaging Software: Works in tandem with microscope software for seamless operation.
  • Hardware Compatibility: Designed to integrate effortlessly with a variety of Zeiss microscope systems.

 

 Benefits of Zeiss Definite Focus System:

  • Enhances Image Quality: Provides sharp, clear images by maintaining constant focus.
  • Reduces Need for Re-Calibration: Saves time and improves efficiency in long-term experiments.
  • Optimized for Live-Cell Imaging: Allows continuous observation without disturbing delicate samples.
  • Compatible with Various Microscopes: Easily integrates with a range of Zeiss microscope models.
  • Automatic Adjustments: Continuously compensates for focus drift during imaging sessions.
  • User-Friendly: Simple to set up and operate, even for novice users.
  • Boosts Productivity: Enables longer uninterrupted imaging sessions without manual intervention.
  • Improves Data Consistency: Yields reliable and reproducible results across multiple experiments.
  • Supports Complex Experiments: Facilitates advanced techniques like time-lapse microscopy.
  • Minimizes Manual Effort: Reduces the workload on researchers by automating routine tasks.
  • Versatile Application Range: Suitable for research in fields like oncology, neurobiology, and developmental biology.
  • Robust Design: Built to withstand rigorous use in professional laboratory environments.

 

This microscope's imaging capabilities:

  • Ability to take still images and live videos of your samples
  • Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
  • Variable linear measurements such as line segment, radius values, and others
  • Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
  • Multi-frame (Time lapse/Image acquisition over time)
  • Multi-slice (Z coordinate/Z Stacking)
  • *Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage

 

We can customize this microscope to fit your requirements:

 

Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)

  • Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)

 

Increase Maximum Magnification and Image Quality:

  • Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
  • We can add standard higher magnification Zeiss microscope objectives 
  • We can add high-end objectives

 

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

  • Additional filter cubes
  • Additional filter sets to match your fluorochromes.
  • Xenon, Metal Halide, or LED Fluorescence microscope light sources

 

We will also ensure the fluorescence light source matches your selected filter sets.

 

Enhance imaging capabilities:

  • We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

 

Add other Motorized/Automation Capabilities:

In addition to installed motorized capabilities, the microscope can be configured

  • Motorized high-speed shutter

 

We can configure it to work with the sample medium you need:

  • Slide, Petri dish, microplate, etc.
  • It may also be set up with a motorized XY stage for multi-slide or full microplate scans.

 

Commonly Requested Customer Upgrades

  • High-End CCD, sCMOS Cameras
  • LED or Metal Halide Fluorescence Light Sources
  • Phase Contrast, Polarization, or the DIC configurations
  • Higher Magnification Objectives
  • Higher performance Fluor or APO objectives
  • Motorized XY Stage (stage, controller, joystick)

 

We are excited to help build and configure the right microscope for you!

 

Zeiss AXIO Observer Z1 LED Fluorescence Definite Focus Microscope Overview 

(Final microscope capabilities will depend on the modifications you request)

 

Suitable for the following applications as configured:

  • Advanced Inverted Microscope: Optimized for detailed live cell imaging in high-end research settings.
  • Definite Focus System: Automatically maintains sharp focus to prevent drift during long-term experiments.
  • Tailored for Cell Biology: Excellently suited for complex neuroscience and developmental biology studies.
  • Infrared Focus Technology: Non-invasive infrared technology is used to stabilize the focal plane, preserving sample integrity.
  • Continuous Imaging: Ideal for extended observations without the need for frequent manual adjustments.
  • Increases Research Productivity: Reduces manual intervention, allowing researchers to focus on analysis.

 

Other potential applications:

  • Phase contrast and DIC: Enhances visibility of transparent specimens without staining.
  • Time-lapse photography: Captures sequential images to monitor changes over time.
  • Multi-dimensional imaging: Allows for the acquisition of images in multiple planes and dimensions.
  • Quantitative analysis: Supports measurements and quantification of specimen features.
  • High-resolution imaging: Provides clear, detailed images for in-depth analysis of samples.
  • Live cell observation: Enables detailed study of live cell dynamics in real-time.

 

 

Video Overview:

 

Check out our microscopy capabilities:

https://www.bostonind.com/our-microscopy-capabilities

 

 

How we pack

 

Why should you buy from us?

 

  • We make sure to match the microscope capabilities to your requirements.
  • We have experience servicing and repairing these microscopes.
  • This microscope goes through an extensive set of service steps before shipping.
  • We offer detailed photos and videos of the microscopes you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Many upgrade and repair parts are in stock.
  • Remote installation and microscope operation training are available.
  • On-site installation may be available in the Boston Area (New England area)
  • Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

 

  • It will work!
  • Continental US Warranty 120 Days & 60 Days International.
  • An extended warranty may be available.
  • Renewable single or multi-year warranty agreements are available.
  • You get after-sales technical support.
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

*SM* Small Crate
200#

 

COMMON QUESTIONS AND ANSWERS BELOW

What steps do you take to verify, service, and confirm the functionality of the Zeiss AXIO Observer Z1 LED Fluorescence Definite Focus Microscope?

We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.

 

  • We confirm the microscope's physical condition.
  • We meticulously inspect and clean the optical path within the microscope.
  • We lubricate mechanical components.
  • We assess light source quality using various fluorescence and non-fluorescence samples.
  • We ensure the stage moves smoothly in both fine and coarse adjustments.
  • We ensure the smooth operation of all mechanical parts.
  • We confirm that all electronic/motorized components are working well.
  • We set up communication between the microscope, installed components, and the software.
  • We test the camera by acquiring sample images.
  • We confirm the responsiveness of any included touchscreens and buttons.
  • We ensure necessary accessories and cables are included.

Can you help me identify the best microscope and configuration for me?

We are here to help.

  • Contact us with your application; we will help you identify the best microscope configuration.
  • We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.

 

 

Can I view the Zeiss AXIO Observer Z1 LED Fluorescence Microscope in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The microscope is fully warranted for parts and labor.
  • The customer should retain all packing materials during the warranty period.

Installation & Training:

Option 1 – In Person

If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.

Option 2 – Remote/Virtual

Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.

Both training options include:

  • Review of the microscope and its capabilities.
  • Microscope and software operation.
  • Review of the recommended maintenance procedures.

We generally recommend these training services to anyone who is new to this microscope model.

What should I watch out for when buying a Zeiss AXIO Observer Z1 Fluorescence with Definite Focus Microscope used/pre-owned from another lab?
  • Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.

 

  • We confirm the microscope's physical condition.
  • We meticulously inspect and clean the optical path within the microscope.
  • We assess light quality using various fluorescence and non-fluorescence samples.
  • We ensure the stage moves smoothly in both fine and coarse adjustments.
  • We ensure the smooth operation of all mechanical components.
  • We confirm that all electronic/motorized components are working well.
  • We set up communication between the microscope, installed components, and the software.
  • We test the camera by acquiring sample images.
  • We confirm the responsiveness of any included touchscreens and buttons.
  • We ensure necessary accessories and cables are included.

We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.

Do you have a Zeiss AXIO Observer Z1 LED Fluorescence Microscope on hand?
  • Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this microscope model elsewhere, possibly for less.

Here are some of the reasons for our price.

  • Our microscope will be fully serviced.
  • It will be customized and configured to your requirements.
  • We may offer longer standard warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each microscope we sell.
  • We have to purchase any missing required accessories to make the microscopes complete.
  • We have to procure and stock service parts to fulfill our warranty.
  • We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
  • We guarantee that the microscope will be functional.

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

How fast will you ship?
  • We can ship within a few days to 2 weeks, depending on your required microscope customization.

Will my microscope be insured during shipping?
  • All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

How do you pack? Have you previously packed and shipped a Zeiss AXIO Observer Z1 Definite Focus Microscope? Do you ship Internationally?

Packing

  • Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile microscopes globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

  • When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your microscope was tested and working the day it left our facility and is covered if there are faults.
Sustainability
  • Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-day Domestic USA and
30-day International
After Sale Support
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies