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- Stock: In Stock
- Brand: Nikon
- Product Code: 61818-V2
For Sale Nikon Ti2-E PFS N-SIM Super-Resolution Inverted Motorized Microscope with Dual Fluorescence Illumination
- Fully Functional
- Serviced & in Stock
- Customized to your requirements
- Includes new fluorescence filters
- Technical sales consultation available
- Continental USA 180 DAYS WARRANTY & 60 DAYS INTERNATIONAL
- Includes remote microscope training session and software overview
Service Highlights
- Optics quality is checked for field distortion and uniformity at the specimen plane
- Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
- Preventive Maintenance (PM) is Completed
Please note The N-SIM module is not currently configured and will require a visit from a Nikon technician to set up. Microscope itself is fully functional and was tested.
This is what you will receive before customizing this microscope:
Standard Nikon Ti2-E Inverted Microscope Configuration:
Microscopy Techniques:
Brightfield and Fluorescence Microscopy
Microscope Base:
- Nikon Ti2-E Inverted Microscope
- TI2-CTRE Controller
Microscope Head:
- TC-T-TS Binocular Head
- (2) CFI 10x/22 Eyepieces
Dual Fluorescence Illumination Paths: (Laser + Widefield)
- N-SIM E illuminator unit
- TI2-LA-BF fluorescence light path w/ TI2-LA-FL light guide adapter
Fluorescence Illumination:
- Nikon LU-N3-SIM laser illumination unit (structured illumination / laser-based system)
- Lumencor Sola Reflective Light Source (widefield fluorescence)
Fluorescence filter cube turret:
- (2) TI2-F-FLT-E Motorized 6-position filter cube turrets
Fluorescence filters:
- Quad SIM 405/488/561/640
- HM Half Mirror
- Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
- Other filters available - please inquire
Emission Filter Wheel:
- TI2-P-FWB-E motorized BA filter wheel
- Installed filters: EM525/50, EM605/70, EM700/75
Brightfield Light Path:
- TI2-D-PD Transmitted Illumination Pillar
Brightfield Illumination:
- TI2-D-LHLED LED Brightfield Light Source
Objective Turret:
- 6-Position Motorized Objective turret
- Perfect Focus System (PFS)
Objectives:
- Nikon Plan Apo λ 10x/0.45
- Nikon S Fluor 20x/0.75
- Nikon Apo 60x/1.40 Oil λS
Condenser:
- TC-C-TC 7-position Mechanical Condenser
- LWD 0.52 Condenser Lens
Stage:
- TI2-S-SE-E Motorized Stage
- MCL Nano-Drive piezo stage controller
- PI E-753 digital piezo controller
- Nikon TI2-S-JS joystick controller
Camera ports:
- 100% Eyes
- 100% Left
- 100% Right
- 50% Eyes / 50% Left
Camera:
- Hamamatsu ORCA-Flash4.0 Monochrome sCMOS Camera
Computer & Software:
- Desktop computer running NIS-Elements AR software with a license dongle.
Accessories:
- Environmental enclosure (draft shield for thermal and airflow stability) (No active temperature or CO₂ control installed)
- Necessary power and communication cables
Please note The N-SIM module is not currently configured and will require a visit from a Nikon technician to set up. Microscope itself is fully functional and was tested.
This configuration includes any 3 of these new filter sets.
- New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
- New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
- New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
- New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
- New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)
Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
- Motorized fluorescence imaging with automated filter switching
- Automated multi-position XY stage scanning (tiling, plate imaging)
- Motorized objective switching via 6-position turret
- Motorized emission filter wheel for rapid channel selection
- Automated fluorescence cube switching (dual turrets)
- Motorized focus drive with Perfect Focus System (PFS) for drift correction
- High-speed Z positioning via piezo nanopositioning (fast Z-stack / SIM-ready)
- Integrated joystick control for manual + assisted navigation
- Computer-controlled acquisition via NIS-Elements (multi-dimensional imaging)
- Dual illumination automation (laser + LED fluorescence switching)
This microscope's imaging capabilities:
- Brightfield and multi-channel fluorescence imaging
- Structured illumination (SIM-capable hardware configuration)
- High-resolution imaging with Plan Apo and oil immersion objectives
- Fast Z-stack acquisition with piezo-driven nanopositioning
- Long-term live imaging with Perfect Focus System (PFS)
- Large-area tile scanning and multi-position imaging via motorized stage
Imaging software highlights:
- NIS-Elements AR for multi-dimensional acquisition (XY, Z, time, channels)
- Automated stage mapping and tile scan stitching
- Hardware synchronization with illumination and filter switching
- Real-time image acquisition and device control
- Support for multi-channel fluorescence workflows
- Preset-based experiment automation and reproducibility
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)
- Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
- Microscope can be configured for magnification between 100x and 600x (with 10x Eyepieces)
- We can add standard higher magnification Nikon microscope objectives
- We can add high-end Nikon Apochromat objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
- Additional filter cubes
- Additional filter sets to match your fluorochromes.
- Xenon, Metal Halide, or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
- We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
We can configure it to work with the sample medium you need:
- Slide, Petri dish, microplate, etc.
Commonly Requested Customer Upgrades
- High-End CCD, sCMOS Cameras
- Phase Contrast, Polarization, or the DIC configurations
- Higher Magnification Objectives
- Higher performance Fluor or APO objectives
We are excited to help build and configure the right microscope for you!
Nikon Ti2-E Inverted Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
- Motorized Ti2-E inverted microscope with controller and precision XY stage for automated positioning and imaging.
- Dual fluorescence illumination combining laser-based and LED light sources for flexible multi-channel imaging.
- Motorized filter turrets and emission filter wheel enable rapid, automated fluorescence channel switching.
- Motorized objective turret with high-performance optics supports high-resolution and multi-magnification imaging.
- Integrated Perfect Focus System maintains stable focus during long or multi-position experiments.
- High-sensitivity sCMOS camera, transmitted LED illumination, and environmental enclosure support stable imaging conditions.
Suited for the following applications as configured:
- Multi-channel fluorescence imaging and quantitative analysis
- High-resolution imaging with oil and high-NA objectives
- Automated tile scanning and large-area sample mapping
- Time-lapse imaging with focus stabilization (PFS)
- Fast Z-stack acquisition using motorized and piezo control
- Structured illumination (SIM-ready) imaging workflows
Other potential applications:
- Routine brightfield imaging and sample documentation
- Fixed-cell fluorescence and immunostaining analysis
- Live-cell imaging with environmental control integration
- High-content screening and plate-based assays
- Microfluidics and perfusion-based experiments
- Materials and thin-film inspection under transmitted or fluorescence light
Video Overview:
REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US
- They want to ensure the microscope is customized to fit their application
- They want to ensure that internal optics are properly cleaned
- They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
- They want to ensure that image quality is verified at the sample point for field distortion and uniformity
- They want to ensure that the fluorescence intensity is verified at the sample point
- They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
- They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
- They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
- They want training to learn how to operate the microscope
- They want a reliable warranty
- They want peace of mind knowing it will be safely and professionally packed for shipping
- We make sure to match the microscope capabilities to your requirements.
- We have experience servicing and repairing these microscopes.
- This microscope goes through an extensive set of service steps before shipping.
- We offer detailed photos and videos of the microscopes you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Many upgrade and repair parts are in stock.
- Remote installation and microscope operation training are available.
- On-site installation may be available in the Boston Area (New England area)
- Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
- Warranty
- An extended warranty may be available.
- You get after-sales technical support.
- Customer references are available.
- The microscope will be complete and working!
- Smooth process from the initial quote to order fulfillment.
*SM* Small Crate
300#
COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW
What steps do you take to verify, service, and confirm the functionality of the Nikon Ti2-E Inverted Microscope?
We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.
- We verified the LED lamp and illumination sources and confirmed proper operation.
- We evaluated the objectives and confirmed image clarity and performance.
- We inspected the motorized XY stage and confirmed smooth and accurate movement.
- We assessed the motorized Z-axis focus and verified precise operation.
- We examined the motorized objective turret and confirmed proper switching between objectives.
- We checked the motorized fluorescence filter turrets and verified correct positioning and operation.
- We validated the Perfect Focus System (PFS) and confirmed stable focus tracking over time.
- We reviewed the camera live feed and verified image clarity and responsiveness.
- We confirmed the functionality of all filter cubes.
Can you help me identify the best microscope and configuration for me?
We are here to help.
- Contact us with your application; we will help you identify the best microscope configuration.
- We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.
Can I view the Nikon Ti2-E Inverted Microscope in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The microscope is fully warranted for parts and labor.
- The customer should retain all packing materials during the warranty period.
Installation & Training:
Option 1 – In Person
If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.
Option 2 – Remote/Virtual
Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.
Both training options include:
- Review of the microscope and its capabilities.
- Microscope and software operation.
- Review of the recommended maintenance procedures.
We generally recommend these training services to anyone who is new to this microscope model.
What should I watch out for when buying a Nikon Ti2-E Inverted Microscope used/pre-owned from another lab?
- Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.
- We confirm the microscope's physical condition.
- We meticulously inspect and clean the optical path within the microscope.
- We assess light quality using various fluorescence and non-fluorescence samples.
- We ensure the stage moves smoothly in both fine and coarse adjustments.
- We ensure the smooth operation of all mechanical components.
- We confirm that all electronic/motorized components are working well.
- We set up communication between the microscope, installed components, and the software.
- We test the camera by acquiring sample images.
- We confirm the responsiveness of any included touchscreens and buttons.
- We ensure necessary accessories and cables are included.
We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.
Do you have a Nikon Ti2-E Inverted Microscope on hand?
- Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this microscope model elsewhere, possibly for less.
Here are some of the reasons for our price.
- Our microscope will be fully serviced.
- It will be customized and configured to your requirements.
- We may offer longer standard warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each microscope we sell.
- We have to purchase any missing required accessories to make the microscopes complete.
- We have to procure and stock service parts to fulfill our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the microscope will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to 2 weeks, depending on your required microscope customization.
Will my microscope be insured during shipping?
- All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you previously packed and shipped a Nikon Ti2-E Inverted Microscope? Do you ship Internationally?
Packing
- Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
- Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile microscopes globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your microscope was tested and working the day it left our facility and is covered if there are faults.
Sustainability
- Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.