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- Stock: In Stock
- Brand: Zeiss
- Product Code: 50167
For Sale Zeiss AXIO Imager 2 Imager.D2 LED Fluorescence Phase Contrast Microscope
- Fully Functional
- Serviced & in Stock
- Customized to your requirements
- Includes new fluorescence filters
- Technical sales consultation available
- Continental USA 180 DAYS WARRANTY & 60 DAYS INTERNATIONAL
- Includes remote microscope training session and software overview
Service Highlights
- Optics quality is checked for field distortion and uniformity at the specimen plane
- Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
- Preventive Maintenance (PM) is Completed
This is what you will receive before customizing this microscope:
Standard Zeiss AXIO Imager.D2 LED Microscope Configuration:
Microscopy Techniques:
Brightfield and Fluorescence Microscopy
Microscope Base:
- Zeiss AXIO Imager.D2 Upright Microscope
Microscope Head:
- Trinocular Head
- (2) Pl 10x/25 Eyepieces
Fluorescence Light Path:
- Base
Fluorescence Illumination:
- EXFO X-Cite 120 Metal Halide Light Source w/ light guide and collimating adapter
Brightfield Illumination:
- LED Lamp w/ built-in power supply
Fluorescence filter cube turret:
- 6-position motorized turret
Fluorescence filters:
- Three filter cubes
- Three Brand New filter sets of either DAPI, FITC, Texas Red, CY5, CY3
- *Other filters available - please inquire*
Brightfield Light Path:
- Base
Objectives:
- 5x/0.25 FLUAR (O#04824)
- 10x/0.30 EC Plan-NEOFLUAR Ph1 (O#04419)
- 20x/0.4 LD Plan-NEOFLUAR Ph2 Korr (O#04738)
- *Other objectives available - please inquire*
Condenser:
- Mechanical Condenser
- Light Rings: Ph1, Ph2
Stage:
- Mechanical XY
Photo / Port Splits:
- 100% Head
- 30% Head / 70% Camera
- 100% Camera
Camera:
- Bestscope 8.3MP color CMOS camera
Computer & Software:
- Computer running applicable camera software
Accessories:
- Communication and power cables
This configuration includes any 3 of these new filter sets.
- New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
- New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
- New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
- New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
- New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)
This microscope's imaging capabilities:
Imaging software highlights:
- Ability to take still images and live videos of your samples
- Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
- Variable linear measurements such as line segment, radius values, and others
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)
- Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
- Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
- We can add standard higher magnification Zeiss microscope objectives
- We can add high-end Zeiss Apochromat objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
- Additional filter cubes
- Additional filter sets to match your fluorochromes.
- Xenon, Metal Halide, or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
- We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
We can configure it to work with the sample medium you need:
- Slide, Petri dish, microplate, etc
- It may also be set up with a motorized XY stage for multi-slide or full microplate scans.
Commonly Requested Customer Upgrades
- High-End CCD, sCMOS Cameras
- LED or Metal Halide Fluorescence Light Sources
- Phase Contrast, Polarization, or the DIC configurations
- Higher Magnification Objectives
- Higher performance Fluor or APO objectives
- Motorized XY Stage (stage, controller, joystick)
We are excited to help build and configure the right microscope for you!
Zeiss AXIO Imager 2 Imager.D2 LED Fluorescence Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
- The Zeiss AXIO Imager.D2 is an upright fluorescence microscope designed for precision imaging in both brightfield and fluorescence modes.
- It features a trinocular head with Pl 10x/25 eyepieces and a mechanical XY stage for accurate sample navigation.
- Fluorescence illumination is provided by an EXFO X-Cite 120 metal halide light source, while brightfield uses an integrated LED lamp.
- A 6-position turret accommodates new DAPI, FITC, and Texas Red filter sets, with additional filter options available.
- Objectives include 5x, 10x Ph1, and 20x Ph2 Korr lenses for a range of magnification and contrast methods.
- The system includes a 10MP CMOS camera and computer with imaging software for capturing and analyzing high-resolution images.
Suitable for the following applications as configured:
- Brightfield and fluorescence imaging of biological samples
- Multi-channel fluorescence imaging using DAPI, FITC, and Texas Red filter sets
- Phase contrast imaging with Ph1 and Ph2 ring-equipped objectives
- Histology and pathology slide review with LED illumination
- Fixed sample analysis in neuroscience, cell biology, or microbiology
- Digital imaging and documentation using the 10MP CMOS camera
Other potential applications:
- Immunofluorescence staining analysis
- Fluorescent protein localization in cells or tissue
- Multicolor fluorescence microscopy with CY3 and CY5 filters
- Educational demonstrations in advanced life sciences
- High-resolution static imaging for publication-quality results
- Pathogen or bacterial detection in clinical research
Video Overview:
REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US
- They want to ensure the microscope is customized to fit their application
- They want to ensure that internal optics are properly cleaned
- They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
- They want to ensure that image quality is verified at the sample point for field distortion and uniformity
- They want to ensure that the fluorescence intensity is verified at the sample point
- They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
- They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
- They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
- They want training to learn how to operate the microscope
- They want a reliable warranty
- They want peace of mind knowing it will be safely and professionally packed for shipping
- We make sure to match the microscope capabilities to your requirements.
- We have experience servicing and repairing these microscopes.
- This microscope goes through an extensive set of service steps before shipping.
- We offer detailed photos and videos of the microscopes you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Many upgrade and repair parts are in stock.
- Remote installation and microscope operation training are available.
- On-site installation may be available in the Boston Area (New England area)
- Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
- Warranty
- An extended warranty may be available.
- You get after-sales technical support.
- Customer references are available.
- The microscope will be complete and working!
- Smooth process from the initial quote to order fulfillment.
*SM* Small Crate
300#
COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW
What steps do you take to verify, service, and confirm the functionality of the Zeiss AXIO Imager 2 Imager.D2 LED microscope?
We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.
- We confirm the microscope's physical condition.
- We meticulously inspect and clean the optical path within the microscope.
- We lubricate mechanical components.
- We assess light source quality using various fluorescence and non-fluorescence samples.
- We ensure the stage moves smoothly in both fine and coarse adjustments.
- We ensure the smooth operation of all mechanical parts.
- We confirm that all electronic/motorized components are working well.
- We set up communication between the microscope, installed components, and the software.
- We test the camera by acquiring sample images.
- We confirm the responsiveness of any included touchscreens and buttons.
- We ensure necessary accessories and cables are included.
Can you help me identify the best microscope and configuration for me?
We are here to help.
- Contact us with your application; we will help you identify the best microscope configuration.
- We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.
Can I view the Zeiss AXIO Imager 2 Imager.D2 LED microscope in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The microscope is fully warranted for parts and labor.
The customer should retain all packing materials during the warranty period.
Installation & Training:
Option 1 – In Person
If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.
Option 2 – Remote/Virtual
Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.
Both training options include:
- Review of the microscope and its capabilities.
- Microscope and software operation.
- Review of the recommended maintenance procedures.
We generally recommend these training services to anyone who is new to this microscope model.
What should I watch out for when buying a Zeiss AXIO Imager 2 Imager.D2 LED microscope used/pre-owned from another lab?
- Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.
- We confirm the microscope's physical condition.
- We meticulously inspect and clean the optical path within the microscope.
- We assess light quality using various fluorescence and non-fluorescence samples.
- We ensure the stage moves smoothly in both fine and coarse adjustments.
- We ensure the smooth operation of all mechanical components.
- We confirm that all electronic/motorized components are working well.
- We set up communication between the microscope, installed components, and the software.
- We test the camera by acquiring sample images.
- We confirm the responsiveness of any included touchscreens and buttons.
- We ensure necessary accessories and cables are included.
We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.
Do you have a Zeiss AXIO Imager 2 Imager.D2 LED microscope on hand?
- Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model microscope elsewhere, possibly for less.
Here are some of the reasons for our price.
- Our microscope will be fully serviced.
- It will be customized and configured to your requirements.
- We may offer longer standard warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each microscope we sell.
- We have to purchase any missing required accessories to make the microscopes complete.
- We have to procure and stock service parts to fulfill our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the microscope will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to 2 weeks, depending on your required microscope customization.
Will my microscope be insured during shipping?
- All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you previously packed and shipped a Zeiss AXIO Imager 2 Imager.D2 LED microscope? Do you ship Internationally?
Packing
- Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
- Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile microscopes globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your microscope was tested and working the day it left our facility and is covered if there are faults.
Sustainability
- Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
Manual for Zeiss AXIO Imager.D2 LED Fluorescence Motorized Phase Contrast Microscope - AV