The Predecessor to Nikon Ti2-E PFS Inverted Fluorescence Microscope
This is what you will receive before customizing this microscope:
Standard Nikon Eclipse Ti-E PFS Inverted Microscope Configuration:
Microscopy Techniques:
Brightfield with Phase Contrast, Fluorescence, and PFS (Perfect Focus automated focal drift control)
Microscope Base:
Nikon Eclipse Ti-E Base
with a Motorized(Automated) Z-Axis Drive
TI-HUBC/A Controller
Microscope Head:
Binocular Head
Two Eyepieces
Fluorescence Light Path:
TI-FL Fluorescence Light Path
Fluorescence Illumination:
Lumencor Sola LED Light Source
Collimating adapter
Light guide
Fluorescence filter cube turret:
TI-FLC-E 6-position Motorized Turret
Fluorescence filters:
Three empty filter cubes
Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
Brightfield Light Path:
Illumination Tower
Brightfield Illumination:
D-LH/LC 12V 100W Halogen Lamp
TE-PS100 power supply
Objective Turret:
TI-ND6-PFS-S 6-position Motorized Nosepiece
Nikon Perfect Focus System (PFS)
Objectives:
4x/0.13 Plan Fluor PhL
10x/0.30 Plan Fluor Ph1
20x/0.75 CFI Plan Apo VC
Condenser:
TI-C Mechanical Condenser w/ LWD 0.52
PhL insert
Ph1 insert
Stage:
Motorized XY Stage
LEP MAC6000 Stage Controller
Joystick
Camera ports:
100% Eyepiece, 0% Left Camera
20% Eyepiece, 80% Left Camera
0% Eyepiece, 100% Left Camera
Camera adapter:
1x C-mount adapter
Camera:
CMOS USB 3.0 10MP Camera
Computer & Software:
Dell laptop computer with Windows 10
Micromanager w/ImageJ analysis software
Camera software
Accessories:
Necessary power and communication cables
This configuration includes any 3 of these new filter sets.
New filter set - DAPI(EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
New filter set - EGFP/FITC/Cy2(EX: 475/30nm, DM: 500nm, EM: 530/40nm)(Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
New filter set - TRITC/Cy3/Dsred(EX: 545/20nm, DM: 562nm, EM: 605/60nm)(Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
New filter set - Texas Red(EX: 560/20nm, DM: 585nm, EM: 630/40nm)(Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm)(Dye compatibility: Cy5/AlexaFluor 647/Draq5)
Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
Motorized lightpath
Motorized/automated Z-Axis Drive
Motorized filter cube turret
Motorized objective(nosepiece) turret
Motorized automated focal drift control (PFS Perfect Focus System)
Motorized XY Stage
Benefits of motorized/Automated Z-Axis drive:
Automatic parfocality adjustment
The ability to recall previously saved focus levels
Automated management of Z positioning/ Fine-coarse control
How the Nikon Perfect Focus System Works
Infrared Light Source: Uses IR light, reducing photodamage and bleaching in live cells.
Dedicated Optical Path: IR light hits the sample via a path that includes a dichroic mirror, allowing visible light through.
Reflected Light Detection: IR light reflection from the cover glass-sample surface is detected.
Focus Offset Detection: Continuous measurement of reflected IR light position to detect focal shifts or drifts.
Automatic Correction: Corrects focus in real-time by adjusting the lens or stage when shifts or drifts are detected.
Benefits of Nikon Perfect Focus System(PFS):
Near Effortless swapping of samples at different focal ranges: Swapping samples with focal planes within the PFS range allows for fast refocusing.
Long-term Observations: Enables the continuous observation of live cells over extended periods.
Minimizing Phototoxicity: By keeping the focus precise, the need for frequent manual adjustments and the associated increased light exposure is reduced.
Automated Multi-well Plate Scanning: Allows for the automated and consistent focus across different wells in a multi-well plate.
Embryo Imaging: Provides the stability needed to image developing embryos over time.
Neuron Activity Studies: The system can maintain focus on specific areas of the brain or neural cultures over long periods.
Reduced Photobleaching: By maintaining focus with minimal adjustments, the PFS helps in reducing exposure to intense light.
Improved Z-stacking: Ensures consistent focus across multiple z-planes.
Stable Time-lapse Sequences: Vital for capturing time-lapse sequences where a consistent focus is required to accurately document changes over time.
Surface Topography Studies: Maintaining focus is essential for accurately mapping surface topographies and understanding material properties.
Drug Response Monitoring: Allows for the detailed observation of cellular responses to drug treatments over time.
This microscope's imaging capabilities:
Imaging software highlights:
Ability to take still images and live videos of your samples
Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
Variable linear measurements such as line segment, radius values, and others
Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
Multi-frame (Time lapse/Image acquisition over time)
Multi-slice (Z coordinate/Z Stacking)
Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield with Phase Contrast and Fluorescence)
DIC or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
We can add standard higher magnification Nikon microscope objectives
We can add high-end NikonApochromat objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
Additional filter cubes
Additional filter sets to match your fluorochromes.
Xenon, Metal Halide, or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
Add other Motorized/Automation Capabilities:
In addition to installed motorized capabilities, the microscope can be configured
Motorized Condenser or Motorized high-speed shutter
We can configure it to work with the sample medium you need:
Slide, Petri dish, microplate, etc
Commonly Requested Customer Upgrades
High-End CCD, sCMOS Cameras
LED or Metal Halide Fluorescence Light Sources
Phase Contrast, Polarization, or the DIC configurations
Higher Magnification Objectives
Higher performance Fluor or APO objectives
We are excited to help build and configure the right microscope for you!
We make sure to match the microscope capabilities to your requirements.
We have experience servicing and repairing these microscopes.
This microscope goes through an extensive set of service steps before shipping.
We offer detailed photos and videos of the microscopes you buy.
We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
Many upgrade and repair parts are in stock.
Remote installation and microscope operation training are available.
On-site installation may be available in the Boston Area (New England area)
Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
Warranty
An extended warranty may be available.
You get after-sales technical support.
Customer references are available.
The microscope will be complete and working!
Smooth process from the initial quote to order fulfillment.
*SM* Small Crate
200#
COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW
What steps do you take to verify, service, and confirm the functionality of the Nikon Eclipse Ti-E PFS microscope?
We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.
We confirm the microscope's physical condition.
We meticulously inspect and clean the optical path within the microscope.
We lubricate mechanical components.
We assess light source quality using various fluorescence and non-fluorescence samples.
We ensure the stage moves smoothly in both fine and coarse adjustments.
We ensure the smooth operation of all mechanical parts.
We confirm that all electronic/motorized components are working well.
We set up communication between the microscope, installed components, and the software.
We test the camera by acquiring sample images.
We confirm the responsiveness of any included touchscreens and buttons.
We ensure necessary accessories and cables are included.
Can you help me identify the best microscope and configuration for me?
We are here to help.
Contact us with your application; we will help you identify the best microscope configuration.
We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.
Can I view the Nikon Eclipse Ti-E PFS microscope in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
The microscope is fully warranted for parts and labor.
The customer should retain all packing materials during the warranty period.
Installation & Training:
Option 1 – In Person
If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.
Option 2 – Remote/Virtual
Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.
Both training options include:
Review of the microscope and its capabilities.
Microscope and software operation.
Review of the recommended maintenance procedures.
We generally recommend these training services to anyone who is new to this microscope model.
What should I watch out for when buying a Nikon Eclipse Ti-E PFS microscope used/pre-owned from another lab?
Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
Confirm that there is no physical damage or any misaligned internal components.
Ask what accessories are included.
Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.
We confirm the microscope's physical condition.
We meticulously inspect and clean the optical path within the microscope.
We assess light quality using various fluorescence and non-fluorescence samples.
We ensure the stage moves smoothly in both fine and coarse adjustments.
We ensure the smooth operation of all mechanical components.
We confirm that all electronic/motorized components are working well.
We set up communication between the microscope, installed components, and the software.
We test the camera by acquiring sample images.
We confirm the responsiveness of any included touchscreens and buttons.
We ensure necessary accessories and cables are included.
We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.
Do you have a Nikon Eclipse Ti-E PFS microscope on hand?
Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model microscope elsewhere, possibly for less.
Here are some of the reasons for our price.
Our microscope will be fully serviced.
It will be customized and configured to your requirements.
We may offer longer standard warranty terms.
We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
We invest considerable time into the testing and service of each microscope we sell.
We have to purchase any missing required accessories to make the microscopes complete.
We have to procure and stock service parts to fulfill our warranty.
We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
We guarantee that the microscope will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
POs are accepted from US and Canadian universities.
How fast will you ship?
We can ship within a few days to 2 weeks, depending on your required microscope customization.
Will my microscope be insured during shipping?
All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you previously packed and shipped a Nikon Eclipse Ti-E PFS microscope? Do you ship Internationally?
Packing
Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
Accessories are packed safely in boxes with padding and then safely packed inside the crate.
We may not use a crate for local deliveries to save you money *if delivered by us.
We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile microscopes globally.
Our experience and process
We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
Know that your microscope was tested and working the day it left our facility and is covered if there are faults.
Sustainability
Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.